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Sperm Membrane Integrity
It helps in understanding the overall fertilization capacity of sperms. Compromised sperm membrane integrity has an effect on: Sperm Motility, Sperm Capacitation, Acrosome Reaction & binding of sperm to the Zona Pellucida.
Sperm Vitality is measurable through Sperm Membrane Integrity assessment and it reflects the proportion of live spermatozoa. Sperms exposed to oxidative stress may have DNA damage and are likely to have compromised plasma membrane; thus, are incapable of natural fertilization.
Functional membrane integrity
Functional membrane integrity is defined as the ability of sperm's outer membrane to maintain equilibrium between the sperm cell contents & surrounding environment. It can be evaluated by response of sperm to osmotic environmental stress.
Intact sperm membrane permits passage of fluid into the Cytoplasmic space in hypo osmotic environment resulting into sperm tail swelling & curling. The pattern of curling may vary from sperm to sperm within the same semen sample.
All the curled tail sperms are considered as Live and Dead Sperms exhibit no cytoplasmic swelling or tail curling (remains straight).
|Semen Analysis Chamber (Sperm Meter)||Semen Collection Container|
|Controlled Temperature 370C Dry bath (Sperm Warmer / Water bath)||Test-tubes|
|Centrifuge Machine (Androspin)||Glass-slides|
|Set of Pipettes||Non-spermicidal Semen Collection Pouch (Sperm Collect)|
|Glass Slide Stand||Coverslips|
|Stop-watch (Andro Watch)||Microtubes / Storage Vials|