Bringing for the first time ever a SFT Kit of Nuclear Protein Assesssment. Now assess levels of Protamine & Histone in the sperm nuclei protein with our ready to use reagent Kit.
Conversion of Histone to Protamine is the most important step in formation of spermatozoa. The mature sperm chromatin contains approximately 85% Protamine & 15% Histone, whereas immature chromatin contains more than 15% Histone.
In 'Aniline Blue' staining, lysine-rich Histone protein in immature nucleoprotein stains as Dark Blue in sperm head region; whereas Protamine (cysteine & arginine rich) in mature nucleoprotein stains as Eosinophilic head region.
Increased levels of persistent Histone indicate a break in the chromatin development sequence: Histone to Transition Protein to Protamine. This significantly affects the DNA packaging, is vulnerable to increased DNA Fragmentation and declined fertilization potential.
Hence, diagnosis & prognosis of male infertility can be determined by Nucleoprotein assessment.
Note : The lysine-rich Histone protein in immature nucleoprotein stains as Dark Blue in sperm head region; whereas Protamine (cysteine & arginine rich) in mature nucleoprotein stains as Eosinophilic head region.
|Semen Analysis Chamber (Sperm Meter)||Semen Collection Container|
|Controlled Temperature 370C Dry bath (Sperm Warmer / Water bath)||Test-tubes|
|Centrifuge Machine (Androspin)||Glass-slides|
|Slide-warmer||Non-spermicidal Semen Collection Pouch (Sperm Collect)|
|Glass Slide Stand||Coverslips|
|Stop-watch (Andro Watch)||Pasture Pipettes|
|Set of Pipettes||Filter Papers|