Products > Sperm Function Test Kits > Nuclear Protein Assay

Conversion of Histone to Protamine is the most important step in formation of spermatozoa. The mature sperm chromatin contains approximately 85% Protamine & 15% Histone, whereas immature chromatin contains more than 15% Histone.  

In 'Aniline Blue' staining, lysine-rich Histone protein in immature nucleoprotein stains as Dark Blue in sperm head region; whereas Protamine (cysteine & arginine rich) in mature nucleoprotein stains as Eosinophilic head region.

Increased levels of persistent Histone indicate a break in the chromatin development sequence: Histone to Transition Protein to Protamine. This significantly affects the DNA packaging, is vulnerable to increased DNA Fragmentation and declined fertilization potential.

Hence, diagnosis & prognosis of male infertility can be determined by Nucleoprotein assessment.

Note : The lysine-rich Histone protein in immature nucleoprotein stains as Dark Blue in sperm head region; whereas Protamine (cysteine & arginine rich) in mature nucleoprotein stains as Eosinophilic head region.

• Store the kit in appropriate conditions as per the instructions given on the package.
• Do not use the kits beyond expiry date given on the package.
• All the reagents are potentially hazardous and it is strictly advised to follow the MSDS instructions.
• All the patients' samples should be treated as potentially infectious. Laboratory personnel are advised to wear protective gloves, face masks, laboratory coats etc while performing the tests.
• Dispose off all the waste generated, during and after the tests, in an appropriate manner as per the governing compliance laws of the land.